Unless you have a really heavy load of calcium scaling, after 24 hours you likely will find the calcium scaling completely gone. It’s been dissolved back into your spa water.
However, your spa water pH is likely too low as vinegar is an acid. If the pH is only a bit lower than the desired 7.2 – 7.6 pH, you can simply adjust it. If it’s quite low, you are likely better off draining and refilling your spa. In either case, remember how you got the calcium scaling. You let the pH get too high – above 8.0.
